By Richard Martin, E. Rand Sutherland
- with authoritative suggestions from 15 professional overseas specialists within the components of pulmonology, microbiology, immunology, allergic reaction, and pediatrics
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Extra info for Asthma and Infections, Volume 238 (Lung Biology in Health and Disease)
The association of viruses with acute asthma. N Z Med J 1987; 100(829):488–490. 30. Mitchell I, Inglis JM, Simpson H. Viral infection as a precipitant of wheeze in children. Combined home and hospital study. Arch Dis Child 1978; 53(2):106–111. 31. Johnston SL, Pattemore PK, Sanderson G, et al. Community study of role of viral infections in exacerbations of asthma in 9–11 year old children [see comments]. BMJ 1995; 310(6989):1225–1229. 32. Nicholson KG, Kent J, Ireland DC. Respiratory viruses and exacerbations of asthma in adults.
Multiplex PCR One of the more encouraging areas of diagnostic procedures to be developed in recent years is the multiplexing assays for respiratory pathogens, especially viruses. Several of these assays have been developed in recent years, which allow the detection of multiple viral nucleic acid targets in a single specimen (47–58). Recently, a multiplex system was described to measure 17 viruses that commonly cause respiratory infections including influenza A and B, parainfluenza virus (PIV) 1, 2, 3, 4a, and 4b, RSV A and B, human rhinovirus (HRV), coronaviruses 229E/NL63 and OC43, adenovirus B, C, and E, and human metapneumovirus (59).
Furthermore, increased sensitivity and specificity are obtained with the added advantage of organism quantitation. Nonamplified Nucleic Acid Probes Nucleic acid probes are segments of DNA or RNA labeled with radioisotopes, enzymes or chemiluminescent reporter molecules that can bind to complementary nucleic acid sequences. These probes can bind with a high degree of specificity. The probes can be designed to identify any microbe. There are a number of commercial kits that can be obtained to be used to directly identify a pathogen in a clinical specimen or one that has been isolated after culture.
Asthma and Infections, Volume 238 (Lung Biology in Health and Disease) by Richard Martin, E. Rand Sutherland